Enterokinase, bovine (E.coli-derived, his-tag): Recombinant Bovine Enterokinase (rbEK )
Source: Escherichia coli Molecular
Weight: Approximately 28 kDa, a single non-glycosylated polypeptide chain containing 241 amino acids with a C-terminal His. Tag.
Quantity: 100IU/250IU/1000IU
Specific activity: ≥ 2 x 104 units/mg recombinant enterokinase.
Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25 mM TrisHCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.
Physical Appearance: Sterile liquid.
Formulation: 50 mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Endotoxin: Less than 1EU/mg of rbEK as determined by LAL method.