Remove lipopolysaccharides (LPS) from plasmid DNA preparations
Quantity
1 volume treats 5 volumes of DNA solution (≤1mg/ml)
Biochem/physiol Actions
The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.