"The ECMatrix substrate has enabled a simpler workflow for human iPSC processing. We save time by eliminating the need to pre-coat our cultureware, while maintaining high-quality pluripotent stem cell cultures" - Research Scientist, Cell Therapy Bioprocessing Group."Human ES cells grown on the ECMatrix substrate have high cell viability, proliferation and were able to differentiate after multiple passages. Additionally, the substrate saves us time by eliminating the need to pre-coat cultureware" - Senior Stem Cell Scientist, Regenerative Medicine Process Development Group.Human pluripotent stem cells (ES and iPS cells) express alpha6beta1 as the major integrin species and therefore can be maintained stably and expanded efficiently in feeder-free conditions on culture vessels coated with it's binding partner laminin-511. However, laminin-511 is not suitable for large-scale production because of its large molecular weight and heterotrimeric nature. Professor Kiyotoshi Sekiguchi's group (Matrixome, Inc.) have solved this problem by producing a recombinant E8 fragment of laminin-511 at large-scale while retaining the full integrin binding activity.
Storage: 2-8C
Application: Xeno-free laminin-511 coating for feeder-free pluripotent stem cell cultures, 350 mug (CHO-S derived)