DISBAC10 is a fluorophore, which can be used in the study of cellular membrane electrical activity. In a resting polarized cell, DISBAC10 resides on the outer leaflet of the membrane where it can accept photons from excited fluorescein-labeled proteins and re-emit the photons at a higher wavelength (fluorescence resonance energy transfer - FRET). Depolarization of the cell causes rapid translocation of DISBAC10 to the inner leaflet of the membrane, thereby increasing the distance between fluorophores and reducing the FRET. DISBAC10 can also be useful with fluorescein-labeled antibodies or specific receptor ligands to visualize specific cell classes.