Synonyms: DFP; DIFP; Diisopropyl phosphorofluoridate; Phosphoric acid diisopropyl ester fluoride
CAS Number: 55-91-4
Formula: C6H14FO3P
Formula Weight: 184.15
Linear Formula: [(CH3)2CHO]2POF
MDL No.: MFCD00008873
Boiling Point: 62 C/9 mmHg(lit.)
Melting Point: -82 C (lit.)
Density: 1.06 g/mL at 25 C(lit.)
Refractive Index: n20/D 1.385(lit.)
Storage: 2-8C
Beilstein Registry Number: 1723307
EC Number: 200-247-6
UNSPSC Code: 12352200
Biochem/physiol Actions: Potent inhibitor of serine proteases such as trypsin and chymotrypsin, and of acetylcholinesterase; also inhibits cathepsin G, cholinesterase, coagulation factor Xa, leucocyte elastase, pancreatic elastase, tissue kallikrein, plasmin, subtilisin, and thrombin. Inhibition of acetylcholinesterase makes this compound especially toxic. Inhibits apoptosis induced by ricin and bacterial toxins.
Analysis Note: Typically used at a concentration of 0.10 mM. A safer alternative inhibitor for serine protease is phenylmethylsulfonyl fluoride (PMSF).
Other Notes: Stored properly at 2-8C, in an unopened bottle, DIFP should be stable for a minimum of two years. DIFPis unstable when exposed to moisture. DIFP will develop a dark yellow color upon decomposition.
RIDADR: UN 3382 6.1 / PGI
WGK Germany: 3
R Codes: 26/27/28
S Codes: 36/37/39-45
IOD Codes: T+
Symbol: GHS06
Signal Word: Danger
Hazard Statements: H300 + H310 + H330
Precautionary statements: P260-P264-P280-P284-P301 + P310-P302 + P350