Synonyms: DCI activator solution
Purity: 29-32 g/L (DCI content, titration)
Other Notes: Activator solution, containing 4,5-dicyanoimidazole (DCI) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5'hydroxyl group of the growing oligonucleotide chain.