This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide (EF1a-dCas9-VP64-2A-Blasticidin) allowing for easy selection following successful transfection or transduction. Use Sigma’s lentiviral dCas9-VP64 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing dCas9-VP64 for CRISPR based transcipritonal activation and genome wide gain of function screening. Sigma’s lenti-dCas9-VP64 plasmid is one part of a three part CRISPR system with individual dCas9-VP64, MS2-p65-HSF1 and gRNA expression vectors. To order gRNA in any format click here
Storage: -20C
Application: Functional Genomics/Target Validation
•Unbiased forward genetic screening
•Strong transcriptional activation in multiple cell lines
•Manufacture of dCas9-VP64 expressing Lentiviral Particles
Legal Information: CRISPR Use License AgreementLentiviral, WPRE and Evrogen Fluorophore Licenses