D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) is a member of the D-2-hydroxyacid NAD+ dependent dehydrogenase family of proteins. D2HGDH catalyzes the conversion of alpha-ketoglutarate (alpha--KG) to D-2-hydroxyglutarate (D2HG), coupled to the oxidation of NADH to NAD+ .The crystal structure of D2HGDH from Acidaminococcus fermentans has been reported. D2HGDH from Acidaminococcus fermentans has been used in several enzymatic assays, such as:
•A continuous spectrophotometric assay to measure the activity of aminotransferases, based on the transamination of a keto compound and L-glutamate, which yields a corresponding amino compound and 2-oxoglutarate.
•Determination of D2HG levels in biological fluids such as serum, urine, cell culture supernatants, and cell or tissue lysates.
•A coupled assay system to measure branched-chain amino acid aminotransferase activity.
Synonyms: D2HGDH; HGDH; L-2-hydroxyglutarate dehydrogenase
Purity: >=95% (SDS-PAGE)
Storage: -20C
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