Application
- The Cytotoxicity Detection KitPLUS (LDH) is a fast, sensitive, and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well or 384-well plate format. The kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example: Detection and quantification of cell-mediated cytotoxicity.
- Determination of mediator-induced cytolysis.
- Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries.
- Determination of cell death in bioreactors.
The assay measures LDH activity released from damaged cells. It is performed in 96-well plates.
Features and Benefits
- Flexible, since the assay can determine the degree of plasma membrane damage in many different in vitro cell systems.
- Safe, because the assay does not use radioactive isotopes.
- Accurate, since assay results strongly correlate to the number of lysed cells.
- Sensitive, since the assay can detect even low cell numbers.
- Fast, since assay results can be determined on a multi-well ELISA reader, which allows simultaneous processing of many samples.
- Convenient, since the assay does not require a prelabeling step.
General Description
Nonradioactive colorimetric assay suitable for high-throughput quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant. The Cytotoxicity Detection KitPLUS (LDH) can be performed in a homogeneous format and requires no transfer and centrifugation steps to separate the supernatant from the cells. The color reaction can be stopped for defined assay conditions.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Usage: sufficient for 2,000 multiwell tests (04744934001); sufficient for 400 multiwell tests (04744926001)
Mfr. No.: Roche
Storage Temp.: −20°C