Cu-immobilized metal affinity chromatography (IMAC) columns consist of Cu-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Cu-IMAC Columns separate proteins via their differences in affinity to Cu2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.
- Imminodiacetate immobilized matrix charged with Cu metal ions.
- IChelating group coupling method: epoxy activation
- IMatrix: cross-linked Separopore® 4B
- IParticle size (range): 50-170µm
- IMetal ion capacity: > 20µmol Cu2+/ml gel
- ISpacer arm: 14 atom
- IpH stability: 3-12
- IBinding Capacity: Approx. 40 mg His-tagged protein/ml medium
- ISupplied as suspension in 20% ethanol.