CSM-MET is a Single dropout formulation of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.
- Dropout supplement for virtually all strains of S. cervisiae
- Add DOB or DOBA Base Medium for vigorous cell growth
- Selects for suxotrophic requirements and transformants
Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM-Met formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7g DOBA powder and 0.77g of CSM-Met to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121°C for 25 minutes. After cooling to 50°C plates were poured.