The Corning Synthegel 3D hiPSC matrix kit is a powerful tool for the physiological formation of 3D hiPSC spheroids in vitro. hiPSC spheroids maintain high viability, pluripotency and differentiation potential based on multiple biomarkers. The kit consists of a vial of Synthegel 3D hiPSC peptide nanofiber solution and a vial of Synthegel X-Link solution. Also required is a vial of lyophilized Synthegel 3D hiPSC Grow Mix.
Synthetic hydrogels for physiologically relevant spheroids and 3D hiPSC culture.
Corning Synthegel 3D matrix kits are chemically defined, self-healing synthetic hydrogel bio-tools for culturing many different types of cells including cancer and stem cells, in a 3D format with robustness and convenience. The Synthegel 3D matrix kits provide negligible variability and a more controlled substrate for in vivo-like 3D cell culture. This platform is a critical tool that is useful for cancer research, stem cell research and drug screening in a 3D format. The Synthegel 3D matrix platform supports culture of human induced pluripotent stem cells (hiPSCs) in a 3D embedded format or in an encapsulation for suspension format. It also supports the culture of physiological cancer spheroids within a defined, tunable, and synthetic peptide matrix devoid of acidic or chilled conditions.
The 3D matrix peptide solution is designed to create an extracellular matrix mimicking hydrogel in one simple mixing step. The Synthegel 3D matrix solution is formulated in standard cell culture media at neutral pH and is stable at room temperature. The hydrogel formation can be initiated by directly mixing the peptide solution with the cross-linking agent along with any cell suspension without extra steps. Cultured cells are easy to harvest. The Synthegel 3D matrix kits provide reproducible results and work with standard biological methodologies.
Features:
- Ideal for formation and growth of physiologically relevant cancer spheroids
- Allows 3D culture and passaging of hiPSCs in embedded and suspension conditions
- The hiPSC Grow Mix supplement provides a complete culture environment for hiPSC 3D culture
- Purified synthetic peptide in neutral pH
- Matrix rigidity is tunable by altering the peptide concentration
- Fast hydrogel formation (5 to 30 min.)
- Works with standard cell culture methodologies