Coomassie® Brilliant Blue R-250 is a fast acting, sensitive dye.
Brilliant Blue R can be used for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates.
Grade: Electrophoresis Grade
Suitability: For Detection of Protein Bands Following Electrophoresis
Key Applications: Staining, Detection of lipids, CMC of detergents, and proteins in solution
Application Areas: Staining; Detection of lipids, CMC of detergents, and proteins in solution
Product Type: Biochemicals
Biochemical Category: Labels, Dyes & Stains
Chemical Class: Labels, Dyes & Stains
Extinction Coefficient (E1%): E1%(587nm) = 785 (methanol)
UV/Visible Absorbance: λ max (ethanol) 585 ± 5 nm
Presentation: Dark Powder
Format: Powder
Solubility: Soluble in water (10 mg/mL), 20 mg/ml in methyl cellosolve, ethanol (10 mg/mL), methanol.
Storage & Handling: Store at Room Temperature (15-30°C).