This medium supports the growth of many spore forming and non-spore forming strict anaerobes. It has the ability to initiate growth of bacteria from very small inocula and to maintain the viability of cultures over long period. It is an excellent medium for the storage of aerobic and anaerobic organisms. It is used for cultivation and maintenance of Clostridia and for determining proteolytic activity of anaerobes. FDA has recommended this medium for enumeration and identification of Clostridium perfringens from foods.
Application: For Cultivation of Aerobes and Anaerobes, Especially Pathogenic Clostridia From Clinical, Food and Water Samples. This Can also Be Used as a Maintenance Medium for Stock Cultures
- Appearance: Brown colored granules
- Color and Clarity of prepared medium: Medium amber colored, clear to slightly opalescent supernatant over insoluble granules
- Reaction: Reaction of 12.5% w/v aqueous suspension at 25°C. pH : 7.2±0.2
- Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours