Synonym: Con A
CAS Number: 11028-71-0
EC Number: 234-258-2
MDL Number: MFCD00071069
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Preparation Note
Highly purified by specific affinity chromatography. Purified by modification of the method of Matsumoto.1
Type: Type VI
Form: lyophilized powder
Potency: <20 μg per mL agglutination activity
Impurities: Carbohydrate, essentially free, salt, essentially free
Solubility: PBS: soluble4 % (w/v)
Storage Temp.: −20°C