Complete Supplement Mixture (CSM) is a culture growth media supplement for virtually all strains of S. cerevisiae.
- Molecular Biology Certified
- Add DOB or DOBA Base to promote vigorous cell growth
Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB (or DOBA) with CSM formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid medium (or plate medium) by adding 2.7g DOB (or 4.37g DOBA) powder and 0.079 g of CSM to a 100 mL of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121°C for 25 minutes. After cooling to 50°C, plates of DOBA with CSM were poured.