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Each kit contains 2x 1L eBlot L1 PVDF Membrane Transfer Buffer (5X), 2x 15mL eBlot L1 PVDF Membrane Equilibration Buffer (10X), 2x 15pK eBlot L1 Transfer Sponge and 2x 15pK eBlot L1 PVDF Membrane. The kit is for 30x transfers with standard program.