Collagenase Type 3 is lower in secondary proteolytic contaminant activities but with typical collagenase activity. It is suggested for mammary primary cell isolation. Collagenase is typically used at concentrations from 0.05 % to 0.5 % (w/v) in balanced salt solutions such as Hank’s, Earle’s and others. For best results the precise mixture of collagenase and proteolytic activities must be tailored to the tissue to be dissociated. Specific conditions for reactivity should be optimized by the end user.