This product is an expression plasmid that utilizes the CMV promoter for strong transient expression of eSpCas9 and GFP linked by a 2A peptide (CMV-eSpCas9-2A-GFP) allowing for easy visualization of successful transfection. The eSpCas9 expression plasmid is one part of a two part CRISPR system with individual eSpCas9 and gRNA expression vectors. To order gRNA in any format click here
Storage: -20C
Application: Functional Genomics/Target Validation
•Creation of gene knockouts in multiple cell lines
•Complete knockout of genes not amenable to RNAi
•Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
Legal Information: CRISPR Use License AgreementEvrogen Fluorophore Licenses