Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at lambdaex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 muM citrate for colorimetric assays and 0.5 to 40 muM for fluorimetric assays.
Storage: -20C