The codA gene encodes the choline oxidase enzyme.
Synonyms: Choline: oxygen 1-oxidoreductase
Storage: -20C
Application: Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.†
Biochem Physiol Actions: Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis helps to transform choline to glycinebetaine (N-trimethylglycine).