Choline oxidase (EC 1.1.3.17) is an enzyme that catalyzes the chemical reaction Choline + O2 betaine aldehyde + H2O2. Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is choline:oxygen 1-oxidoreductase. This enzyme participates in glycine, serine, and threonine metabolism. It employs one cofactor, FAD.
The enzyme is useful for enzymatic determination of phospholipids by coupling with phospholipase D and for cholinesterase activity assays. Choline oxidase from Alcaligenes sp. has been used in a study to investigate a choline biosensor constructed with chitinous membrane and its application in measuring cholinesterase inhibitory activities. Choline oxidase from Alcaligenes sp. has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.
Choline oxidase from Alcaligenes sp. has been used to evaluate choline biosensors constructed with chitinous membranes. The enzyme has also been used to analyse the photoreaction of choline oxidase with covalently bound flavin, using spectroscopic techniques. Furthermore, the product can be used for cholinesterase activity and phospholipid determination assays.
One unit causes the formation of one µmole of hydrogen peroxide per minute at pH 8.0 at 37°C.
Specific Activity: >10 units/mg solid
Key Applications: Catalyst
Product Type: Proteins, Enzymes & Peptides
Presentation: Yellowish powder
Format: Lyophilized powder
Isoelectric point (pI): 4.1 ± 0.1
Solubility: Dilute in 10 mM Tris-HCl buffer, pH 8.0 containing 2 mM EDTA and 1.0% KCl.
Storage & Handling: -20°C