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HiMedia

Certified Tryptone

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Tryptone, Certified is manufactured by a controlled enzymatic hydrolysis of Casein. It is rich in tryptophan and used by a wide variety of microorganisms, recommended in a number of media such as Sterility Testing media, Diagnostics Media, media for biochemical characterization etc.

Appearance
Off white to light yellow Homogenous Free flowing powder, having Characteristic odour but not putrescent.

Solubility
Freely soluble in distilled/purified water, insoluble in alcohol and ether.

Clarity
1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes.

Reaction
Reaction of 2% w/v aqueous solution at 25ºC.

Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.

pH6.30- 7.30
Total aerobic microbial count (cfu/gm)By plate method when incubated at 30-35°C for not less than 3 days.
Bacterial Count : <= 2000 CFU/gram
Total Yeast and mould count (cfu/gm)By plate method when incubated at 20-25°C for not less than 5 days.
Yeast & mould Count : <= 100 CFU/gram
Test for Pathogens1. E.coli-Negative in 10 gms of sample 2. Salmonella species-Negative in 10 gms of sample 3. Pseudomonas aeruginosa-Negative in 10 gms of sample 4. Staphylococcus aureus-Negative in 10 gms of sample 5. C.albicans- Negative in 10 gms of
sample 6. Clostridia- Negative in 10 gms of sample
Indole TestTryptophan content: Passes
Cultural responseCultural response observed after an incubation at 35-37ºC for 18-48 hours by preparing Soyabean Casein Digest Medium (M011) using Tryptone, Certified as an ingredient.

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Product Detail
Thomas No.
C985G27
Mfr. No.
CR014-100G
Description
Tryptone, Certified, 100 g
list price/quantitytotal
$0.00
$0.00 (0 Items)
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