Storage: -20C
UNSPSC Code: 12352200
General description: The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.
Application: NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.
Linkage: Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549
Other Notes: Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
Components: 10x Lysis Buffer, Hypotonic 7 mL; 3x Dilution and Equilibration Buffer 90 mL; 1 M DTT .4 mL; Extraction Buffer 10 mL; IGEPAL(R) CA-630 10% Solution 4 mL; 5x Lysis Buffer, Isotonic 14 mL; Protease Inhibitor Cocktail 1 mL
RIDADR: UN 3316 9
Flash Point(F): 188.6 F
Flash Point(C): 87 C
R Codes: 35
S Codes: 26-36/37/39-45
IOD Codes: C
Symbol: GHS05, GHS09
Signal Word: Danger
Hazard Statements: H290-H314-H410
Precautionary statements: P273-P280-P303 + P361 + P353-P304 + P340 + P310-P305 + P351 + P338 + P310-P391