Carcinoembryonic antigen (CEA) also known as Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5), CD antigen CD66e, Meconium antigen 100, is an oncofetal glycoprotein that is normally expressed by mucosal cells. CEA is a member of the immunoglobulin (Ig) superfamily of proteins. CEA is a glycophosphatidylinositol- (GPI-) linked membrane-anchoring protein that is exposed to the cell surface that faces the extracellular matrix. The membrane-anchoring region of CEA can be cleaved by phospholipase C and phospholipase D. The cleaved products are soluble and circulating through blood vessels. Thus, CEA can be present as secreted and cell surface-anchored forms. CEA is functionally associated with cellular interaction, cell adhesion, immune response, anoikis resistance, and promotion of liver metastasis. CEA overexpression is associated with many types of cancers including gastrointestinal, respiratory, and genitourinary system and breast cancers.
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Synonyms CEACAM5; CD66e; Meconium antigen 100
Description Carcinoembryonic antigen (CEA) also known as Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5), CD antigen CD66e, Meconium antigen 100, is an oncofetal glycoprotein that is normally expressed by mucosal cells. CEA is a member of the immunoglobulin (Ig) superfamily of proteins. CEA is a glycophosphatidylinositol- (GPI-) linked membrane-anchoring protein that is exposed to the cell surface that faces the extracellular matrix. The membrane-anchoring region of CEA can be cleaved by phospholipase C and phospholipase D. The cleaved products are soluble and circulating through blood vessels. Thus, CEA can be present as secreted and cell surface-anchored forms. CEA is functionally associated with cellular interaction, cell adhesion, immune response, anoikis resistance, and promotion of liver metastasis. CEA overexpression is associated with many types of cancers including gastrointestinal, respiratory, and genitourinary system and breast cancers.
Species Human
Source CHO 3E7
Tag C-terminal 8×His Tag.
Biological Activity Immobilized CEA, His, Human at 1 μg/ml (100 μl/well) can bind Human CEA Antibody (1A5C3), Mouse with EC50=2.323 ng/ml when detected by M6 Goat Anti Mouse Fc.
Sequence Accession#P06731 (Lys35-Ala685)
Properties
- Purity > 95% as analyzed by reducing SDS-PAGE
- Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
- Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
- Endotoxin Level < 1 EU/μg, determined by LAL method.
- Storage Lyophilized recombinant Human CEA remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human CEA should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
- Note For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.