Catalase from bovine liver, lyophilized powder, =10,000 units/mg protein

Synonym: H₂O₂:H₂O₂ oxidoreductase

CAS Number: 9001-05-2

EC Number: 232-577-1

Enzyme Commission (EC) Number: 1.11.1.6

MDL Number: MFCD00081483

Analysis Note
Protein determined by biuret

Application
Catalase is used to eliminate H₂O₂ from enzymatic oxidation reactions.^1,2

Biochem/physiol Actions
Activates the decomposition of hydrogen peroxide, Natural antioxidant used to study roles of reactive oxygen species in gene expression and apoptosis.

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen.

Catalase from bovine liver catalyzes the decomposition of H₂O₂ into water and oxygen.^1,2

Caution
Freezing of solutions is not recommended.

Unit Definition
One unit will decompose 1.0 µmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Physical properties
Catalase from bovine liver is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group.