This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and RFP linked by a 2A peptide (EF1a-Cas9-2A-RFP) allowing for easy visualization of successful transfection or transduction. Use Sigma's lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma's Cas9-RFP lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed when packaged into lentiviral particles. To order gRNA in any format click here
Storage: -20C
Application: Functional Genomics/Target Validation
•Creation of gene knockouts in multiple cell lines
• Complete knockout of genes not amenable to RNAi
•Manufacture of Cas9-RFP expressing Lentiviral Particles
Legal Information: CRISPR Use License AgreementLentiviral, WPRE and Evrogen Fluorophore Licenses