Ready-to-use Cas9-GFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-GFP lentiviral particles efficiently and stably integrate Cas9 and GFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed. To order gRNA in any format click here
Storage: -70C
Application: Functional Genomics/Target Validation
•Creation of gene knockouts in multiple cell lines
•Complete knockout of genes not amenable to RNAi
•Creation of cell lines stably expressing Cas9-GFP
Legal Information: CRISPR Use License AgreementEvrogen Fluorophore Licenses