Burk's Medium is used for isolation and cultivation of nitrogen fixing bacteria such as Azotobacter species from soil. This medium contains inorganic salts along with carbohydrate source but lacks nitrogen source. Nitrogen fixing bacteria are able to fix atmospheric nitrogen and grow when cultured on this nitrogen-free medium.
- Appearance: White to cream homogeneous free flowing powder
- Color and Clarity of prepared medium: Colorless clear solution over a white precipitate
- Cultural characteristics after an incubation at 30°C for 7 days