Burkholderia Cepacia Agar Base is used for isolation of Burkholderia cepacia from the respiratory secretions of patients with cystic fibrosis and other non-clinical specimens. The medium is selective for B. cepacia due to bile salts, crystal violet and antibiotics such as Polymyxin B and Gentamicin. Burkholderia cepacia can be identified as sage green colored colonies with bright pink colored medium.
- Appearance: Light yellow to pink homogeneous free flowing powder
- Gelling: Firm, comparable with 1.2% Agar gel
- Color and Clarity of prepared medium: Orange colored clear to slightly opalescent gel forms in Petri plates
- Reaction of 3.65% w/v aqueous solution at 25°C. pH : 6.2±0.2
- M1640: Cultural characteristics observed, with addition of Burkholderia Selective Supplement (FD232), after an incubation at 35-37°C for 48-72 hours.