Bsu DNA Polymerase. Large fragment is from Bacillus subtilis and is modified to retain its 5’→ 3’ DNA polymerase activity with its 5’→ 3’ exonuclease domain removed. It also lacks the 3’→ 5’ exonuclease activity.
Applications
- Second strand cDNA synthesis
- Strand displacement DNA synthesis
- Random primer labeling
- dA-tailing
Product Source
E. coli strain expressing Bsu DNA Polymerase, Large fragment gene.
Product Includes
- Bsu DNA Polymerase, Large fragment
- 10X Bsu DNA Polymerase, Large fragment reaction buffer
10X Bsu DNA Polymerase, Large fragment
100 mM Tris-HCl
500 mM KCl
100 mM MgCl2
10 mM DTT, pH 7.9 @ 25°C
Storage Buffer
50mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol, pH 7.5 @ 25ºC
Storage Temperature: – 20ºC
Heat Inactivation: 65°C for 20 min
Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 37º C.
Quality Control assays
Bsu DNA Polymerase, Large fragment is free from detectable nuclease contamination.