G-bio BL21 (DE3) chemical competent cell is a widely used E. coli strain. It is suitable for protein expression under T7 promoter.
Features
• T7 Expression Strain
• Deficient in proteases Lon and OmpT
• Resistance to phage T1 (fhuA)
• Free of animal products)
Genotype
fhuA2 [lon] ompT gal (λ DE3) [dcm] ?hsdS λ DE3 = λ sBamHIo ?EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ?nin5
Transformation efficiency: 1-2 x 107 cfu/µg pUC19 DNA