Synonym: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide
CAS Number: 23491-52-3
Empirical Formula (Hill Notation): C27H28N6O · 3HCl · xH2O
Molecular Weight: 561.93 (anhydrous basis)
Beilstein Registry Number: 1234011
MDL Number: MFCD00012678
PubChem Substance ID: 24891639
Application
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
Biochem/physiol Actions
Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Packaging
1 g in glass bottle; 100, 500 mg in glass bottle; 25 mg in poly bottle
Quality Level : PREMIUM
Assay: =98% (HPLC and TLC)
Solubility: H2O: soluble20 mg/mL; phosphate buffer: precipitates
Suitability: suitable for fluorescence
Storage Temp.: -20°C