beta-galactosidase, also called beta-gal or beta-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides through the breaking of a glycosidic bond. beta-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. beta-gal has also been used to measure cellular senescence (SA-beta-gal). The BioTracker 543 Yellow beta-Gal Dye is a fluorescent probe for the detection of beta-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. Because the dye is almost non-fluorescent in the absence of beta-galactosidase, this probe exhibits good S/N ratio.Spectral PropertiesAbsorbance: 525nm (488nm compatible)Emission: 543nm
Synonyms: Live cell imaging probe