Synonyms: N-(2-((3-(4-Hydroxyphenethylamino)-3-oxopropyl)disulfanyl)ethyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide; Cleavable biotin-phenol; Cleavable biotin-tyramide linker
Molecular Formula: C23H34N4O4S3
Molecular Weight: 526.74
Linear Structural Formula: C23H34N4O4S3
Purity: >=95%
Storage: -20C
Application: Biotin-SS-tyramide is a cleavable, biotinylated reagent for tyramide signal amplification used in many applications including immunohistochemistry, in situ hybridization, electron microscopy, ELISA, and others. It can be used together with both chromogenic and fluorescence visualization methods. The linker can also be used for synthesis of chemical biology tools for labeling target proteins in biological experiments and assays. It possesses dual functionality: enrichment via the biotin and an embedded disulfide bridge for cleavage under reducing conditions or by glutathione in the lysosome.
Other Notes: Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activityNew Insights into the DT40 B Cell Receptor Cluster Using a Proteomic Proximity Labeling AssayA Catalysis-Based Selection for Peroxidase Antibodies with Increased Activity