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Jackson Immunoresearch

Biotin-SP (long spacer) ChromPure Human IgG, whole molecule

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Biotin-SP (long spacer) ChromPure Human IgG, whole molecule

ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.

Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.

Host: Human

Product: IgG, whole molecule

Conjugate: Biotin-SP (long spacer)

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityBased on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against goat anti-human whole serum is the same as that against goat anti-human IgG, Fc fragment specific. No precipitin line was detected against goat anti-human IgM, Fc5m fragment specific or goat anti-human IgA, a chain specific. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Preservative0.05% Sodium Azide 

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Product Detail
Thomas No.
C840E46
Mfr. No.
009-060-003
Description
Biotin-SP (long spacer) ChromPure Human IgG, whole molecule, 1 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
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