Bile Salt Mixture is recommended for use is microbiological culture media for selective isolation and cultivation of bile tolerant enteric bacteria.It is recommended for use in MacConkey Agar, SS Agar, Violet Red Bile Agar. It selectively inhibits gram-positive and spore bearing microorganisms without interfering with the growth of enteric bacilli. In MacConkey Agar, Violet Red Bile Agar and SS Agar, very little precipitate is observed around coliform colonies facilitating the detection of lactose nonfermenting colonies.
Bile Salts Mixture is obtained by hydrolysis of bile salts. It is equivalent to Bile Salt No.3,for use in Bacteriological culture media as selective inhibitory agent.It is a white coloured, free flowing, fine powder. It is freely soluble in water and forms a colourless, clear solution that produces foam if shaken strongly.
Appearance
White to cream Homogenous Free flowing powder,having Characteristic odor but not putrescent.
Solubility
Soluble in distilled water, insoluble in alcohol and ether.
Clarity
1% aqueous solution is light straw coloured, clear and free from extraneous matter.
Reaction
Reaction of 1% w/v aqueous solution at 25ºC.
Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.