BCIP (5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt) is the most widely used chromogenic phosphatase substrate, which forms a dark blue (λmax 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride), which facilitates the precipitation.
- White solid soluble in water
- Store at 4°C and protect from light
- C8H4BrClNNa2O4P
- MW: 370.5
- [102185-33-1]