beta-Glucuronidase from Helix pomatia, Type H-3AF, aqueous solution, >=60,000 units/mL

Synonyms: beta-D-Glucuronide glucuronosohydrolase

CAS Number: 9001-45-0

MDL No.: MFCD00130629

Storage: 2-8C

Enzyme Commission (EC) Number: 3.2.1.31  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Application: β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Biochem/physiol Actions: Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Biochem/physiol Actions: β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail. Optimal pH: . Glucuronidase activity: 4.5 to 5.0 . Sulfatase activity: ~6.2Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone

Linkage: A further processing of Type H-3 to remove agglutinin.

Physical form: Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Quality: Many beta-glucuronidases derived from mollusks also contain sulfatase activity.Some preparations have been reported to contain acid phosphatase activity.

Unit Definition: One Sigma or modified Fishman unit will liberate 1.0 mug of phenolphthalein from phenolphthalein glucuronide per hr at 37 C at pH 5.0 (30 min assay).

Unit Definition: Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 mumole p-nitrocatechol sulfate per hr at pH 5.0 at 37 C.