beta-Glucuronidase from Escherichia coli, >20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

Synonyms: beta-D-Glucuronide glucuronosohydrolase

CAS Number: 9001-45-0

MDL No.: MFCD00130629

Storage: -20C

Enzyme Commission (EC) Number: 3.2.1.31  ( BRENDA | IUBMB )

EC Number: 232-606-8

UNSPSC Code: 12352204

General description: +¦-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.

Application: beta-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Application: +¦-Glucuronidase was used in the study of Cer-+¦-glucuronide synthesis and evaluation of +¦-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.

Biochem/physiol Actions: beta-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Physical form: Highly purified solution in 50% glycerol

Physical form: beta-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Unit Definition: One Sigma or modified Fishman unit will liberate 1.0 mug of phenolphthalein from phenolphthalein glucuronide per hr at 37 C at the pH 6.8 (30 min assay).