This medium is formulated in accordance to FDA and is a modification of Antibiotic assay Medium No.1. This medium is used to develop incoulum of Bacillus subtilis for antibiotic assay.
Essential nutrients,vitamins, mineral, trace elements and growth factors are supplied by peptic digest of animal tissue, pancreatic digest of casein, yeast extract and beef extract. Dextrose in the medium serves as the carbon source for stimulating the growth of the test microorganism. Manganese sulphate in this medium facilitates the sporulation and growth of Bacillus subtilis, which is generally used as test organisms for plate assay of Dihydrostreptomycin and Vancomycin.
Storage and Shelf-life:
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.