Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1µg/mL for WB; CACO2 and PC3 with PFA and MeOH @ 10µg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.