Zinc finger MYND domain-containing protein 11 (UniProt Q8R5C8) is encoded by the Zmynd11 gene (Gene ID 66505) in murine species. ZMYND11/BS69 functions as a co-repressor of adenovirus E1A and cellular transcription factors including the c-Myb and ETS-2 oncoproteins. ZMYND11 contains several histone reader motifs, including a plant homeodomain (PHD), a bromodomain, and a PWWP domain. Lys36-trimethylated histone H3 (H3K36me3) is deposited onto the nucleosomes in the transcribed regions after RNA polymerase II elongation, and ZMYND11 is found to specifically recognize H3K36me3 and regulate RNA polymerase II elongation. Binding study showed that the combined PHD-bromo-PWWP (PBP) module mediates ZMYND11 recognition of H3K36me3 and PWWP domain alone displays little to no affinity toward H3K36me, H3K36me2, or unmethylated H3K36. ZMYND11 is a tumor suppressor that functions as an unconventional transcription co-repressor by modulating RNA polymerase II at the elongation stage. Low expression levels of ZMYND11 in breast cancer patients correlate with poor prognosis. Consistently, ZMYND11 overexpression suppresses cancer cell growth in vitro and tumor formation in mice in vivo.
Synonyms: Zinc finger MYND domain-containing protein 11
Application: Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected a downregulated ZMYND11 level in human osteosarcoma U2OS cell nuclear extracts following ZMYND11 shRNA treatment (Courtesy of Dr. Hong Wen, UT M.D. Anderson Cancer Center, TX).Chromatin Immunoprecipitation Analysis: A representative lot detected ZMYND11 occupancy of c-Myc gene by ChIP using human osteosarcoma U2OS cell nuclear extracts. ZMYND11 shRNA treatment greatly reduced the c-Myc gene fragments in ChIP (Courtesy of Dr. Hong Wen, UT M.D. Anderson Cancer Center, TX).Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected ZMYND11 chromatin occupancy by ChIP using chromatin preparations from human osteosarcoma U2OS cells. Decreasing H3K36me3 level by SETD2 knockdown reduced ZMYND11 target genes association (Wen, H., et al. (2014). Nature. 508(7495):263-268).ChIP-seq Analysis: A representative lot detected ZMYND11-targeted chromatin sites by a genome-wide ChIP-seq analysis using chromatin preparations from human osteosarcoma U2OS cells. The ZMYND11 occupied sites are highly enriched in introns and exons, but not promoters of targeted genes (Wen, H., et al. (2014). Nature. 508(7495):263-268).Western Blotting Analysis: A representative lot detected exogenously expressed human BS69 protein in yeast and QT-6 quail fibroblasts (Ladendorff, N.E., et al. (2001). Oncogene. 20(1):125-132).
Other Notes: Concentration: Please refer to lot specific datasheet.