Anti-gamma-Tubulin antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

Synonyms: Anti-gamma-Tubulin

Storage: -20C

UNSPSC Code: 12352203

General description: Gamma-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. Gamma-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the beta-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the alpha-tubulin half exposed at the plus end. gamma-Tubulin abundance is less than 1% of the level of either alpha- or beta-tubulin.

Application: Anti-gamma-Tubulin polyclonal antibody is suitable for immunoblotting of materials such as A431 cultured cells whole extract (g-tubulin, 48 kDa), and immunocytochemical staining of cells such as cultured chicken fibroblast cells.

Immunogen: synthetic peptide corresponding to the N-terminal region of human gamma-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for gamma-tubulin and not found in other members of the tubulin family such as alpha- beta-, delta and epsilon- tubulins. This sequence is identical in mouse and rat gamma-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast gamma-tubulin).

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Specificity: Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).