This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. While the predicted molecular weight for TrkCT1 is approximately 68 kDa, the protein has been reported to migrate at about 95 kDa on western blots. The molecular weight noted for TrkCT1 depends somewhat upon the cell lysate or extract used for western blotting, and this variation may be due to post-translational modifications of the protein.