This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Reactivity in other immunoassays is unknown. A HeLa whole cell lysate or mouse testis lysate are suitable for use as a positive control. This product was assayed by western blotting after immunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1:5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 µL for immunoprecipitation.