Anti-Splicing Factor SC-35 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SC-35 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a RBF-DHJ mouse immunized with partially purified spliceosomes prepared from HeLa cells. Splicing Factor SC-35, also known as serine/arginine rich splicing factor 2 (SRSF2) belongs to the family of of serine/arginine-rich (SR) proteins.
Synonyms: Anti-PR264; Anti-SFRS2; Anti-SFRS2A; Anti-SRp30b; Anti-Splicing component 35 kDa (SC35); Anti-serine/arginine-rich splicing factor 2 (SRSF2)
Storage: -20C
Application: Anti-Splicing Factor SC-35 antibody has been used in
•dot blot
•enzyme linked immunosorbent assay (ELISA)
•immunofluorescence
•immunohistochemistry
•immunoblotting
•immunoprecipitation
Biochem Physiol Actions: Splicing Factor SC-35 are critical regulators of constitutive and alternative pre-mRNA splicing. It also controls cellular apoptosis in response to genotoxic stress. Serine/arginine protein kinase 1 (SRPK1) specifically phosphorylates SC-35, resulting in p53 suppression and cyclin D1 upregulation. Elevated levels of SC-35 correlate with aggressive phenotype in several cancer types including lung squamous cell carcinoma and lung adenocarcinoma. SC-35 has oncogenic function in tumor progression of human papilloma virus (HPV)-infected cells.