Sortilin-related receptor (UniProt Q92673; also known as Gp250, LDLR relative with 11 ligand-binding repeats, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LR11, SorLA, SorLA-1, Sorting protein-related receptor containing LDLR class A repeats) is encoded by the SORL1 (also known as C11orf32) gene (Gene ID 6653) in human. SorLA and Sortilin are related Vps10p-domain (Vsp10p-D) family multi-ligand-binding receptors that target LPL, growth factors and peptides, as well as soluble amyloid precursor proein (sAPP) for endocytosis. Studies show that SorLA protects sAPP against proteolytic processing in compartments localized in the cell body (soma) of hippocampal neurons, whereas sortilin facilitates sAPP degradation by lysosomes localized in neurites. Hippocampal neurons lacking sortilin display reduced sAPPalpha production, suggesting that sortilin promotes alpha-secretase activity in neuronal systems. Unlike LRP1 and LRP1b, two other known sAPP-binding receptors, SorLA and Sortilin bind sAPP in a KPI domain-independent manner and their scavenging function therefore is not limited to sAPP produced from KPI-containing APP isoforms. Human SorLA is a single transmembrane (a.a. 2138-2158) protein with a 55-amino acid cytoplasmic tail (a.a. 2159-2214) and a large luminal/extracellular (a.a. 1-2137) region that contains a Vps10p-domain (Vsp10p-D; a.a. 1-731) with a signal peptide (a.a. 1-28) and a propeptide (a.a. 29-81) sequence at the N-terminal end and five BNR (bacterial neuraminidase repeat or (F/Y)WTD/b-propeller domain) repeats (a.a. 136-573). The C-terminal half of the luminal/extracellular region contains five LDL-R class B (LB) repeats (a.a. 800-1013), an EGF-like domain (a.a. 1026-1072), eleven LDL-R class A (LA) repeats (a.a. 1076-1551), and six fibronectin type-III repeats (a.a. 1557-2118).
Synonyms: Sortilin-related receptor, Gp250, LDLR relative with 11 ligand-binding repeats, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LR11, SorLA, SorLA-1, Sorting protein-related receptor containing LDLR class A repeats
Application: Immunocytochemistry Analysis: 5 µg/mL from a representative lot immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes (Courtesy of Dr. Jakob V. Larsen, MIND-centre, University of Aarhus, Denmark). Immunocytochemistry Analysis: A representative lot detected SorLA immunoreactivity primarily located at the cell body (soma) by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized primary murine hippocampal neurons (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).Immunocytochemistry Analysis: A representative lot immunostained endocytic vesicular structures containing endocytosed sAPP by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells expressing exogenously transfected human SorLA (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).Immunocytochemistry Analysis: A representative lot detected the cellular localization of exogenously expressed wild-type and the FANSHY-to-6A4 mutant human SorLA by fluorescent immuncytochemistry staining of 4% paraformaldehyde-fixed, 0.5% saponin-permeabilized SH-SY5Y transfectants (Fjorback, A.W., et al. (2012). J. Neurosci. 32(4):1467-1480).Western Blotting Analysis: A representative lot detected endogenous SorLA in sucrose gradient-fractionated mouse brain extracts as well as the expression of exogenously transfected human SorLA in HEK293 transfectants (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).ELISA Analysis: Representative lots were employed as the detection antibody for quantiating SorLA level in human brain cortical extracts as well as the level of exogenously expressed human SorLA in CHO cells (Caglayan, S., et al. (2012). Arch. Neurol. 69(3):373-379; Schmidt, V., et al. (2012). EMBO J. 31(1):187-200).
Other Notes: Concentration: Please refer to lot specific datasheet.