KH domain-containing, RNA-binding, signal transduction-associated protein 1 (UniProt Q60749; also known as GAP-associated tyrosine phosphoprotein p62, Khdrbs1,p68, p21 Ras GTPase-activating protein-associated p62, Sam68, Src-associated in mitosis 68 kDa protein) is encoded by the Khdrbs1 gene (Gene ID 20218) in murine species. Sam68 is a member of the "signal transduction and activation of RNA" (STAR) family of KH domain-containing RNA-binding proteins that mediates pre-mRNA splicing, mRNA export, and protein translation. It binds to UAAA, UUUA, and UUUUUU motifs with high affinity. RNA immunoprecipitation (RIP) techniques using lysates from UV cross-linked NIH 3T3 cells have identified 23 Sam68 complex-associated mRNA species, including Thrap1, bri3bp, Myl6, Copeb/KLF6/Zf9, Hmgn1, Smc6, and biglycan. In addition, Sam68 is found to be a key regulator of Nrxn1 alternative splicing in the central nervous system in a neuronal activity-dependent manner. Sam68 is phosphorylated on three tyrosine residues (pTyr435, pTyr440, and pTyr443) within the C-terminal nuclear localization signal (NLS) region by the breast tumor kinase (BRK) following EGF stimulation of breast tumor cells with pTyr440 being a principal modulator of Sam68 nuclear localization. Studies comparing wild-type and Sam68-/- knockout mice revealed a positive regulatory role of Sam68 toward adipocyte differentiation, as well as a negative impact of Sam68 against osteoblast differentiation among aged mice.
Synonyms: KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, Sam68, p21 Ras GTPase-activating protein-associated p62, p68
Application: Western Blotting Analysis: 1:500-1:10,000 of a representative lot detected Sam 68 in RIPA lysates from HeLa cells.Immunohistochemistry Analysis: A 1:600 dilution from a representative lot detected Sam 68 in mouse colon tissue (Data courtesy of Stephane Richard, McGill University).Western Blotting Analysis: A representative lot detected Sam 68 in endogenous Sam 68 and exogenously expressed GFP-Sam 68 fusion in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033).Western Blotting Analysis: A representative lot detected Sam 68 in HEK293 transfected with GFP-Sam68, mouse cortex, hippocampus, cerebellum, and mouse brain (Iijima, T., et al. (2011). Cell. 147(7):1601-1614).Western Blotting Analysis: A representative lot detected Sam 68 in HeLa cells transfected with GFP-Sam68, MDA-MB-231, MDA-MB-468, and BT20 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).Western Blotting Analysis: A representative lot detected Sam 68 in lysates from isolated mouse cells and mouse embryonic cell (Richard, S., et al. (2005). PLoS Genet. 1(6):e47).Immunohistochemistry Analysis: A representative lot detected Sam 68 in mouse cerebellum, parasagittal section of brain stem and cerebellum (Iijima, T., et al. (2011). Cell. 147(7):1601-1614).Immunohistochemistry Analysis: A representative lot detected Sam 68 in embryonic mice (Richard, S., et al. (2005). PLoS Genet. 1(6):e47).Immunocytochemistry Analysis: A representative lot detected Sam 68 in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033).Immunoprecipitation Analysis: A representative lot immunoprecipitated Sam 68 in HeLa, HeLa cells transfected with GFP-Sam68, and MDA-MB-468 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).
Other Notes: Concentration: Please refer to lot specific datasheet.