Rootletin (UniProt: Q8CJ40; also known as Ciliary rootlet coiled-coil protein) is encoded by the Crocc (also known as Kiaa0445) gene (Gene ID: 230872) in murine species. Rootletin is a major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells that originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. It contributes to centrosome cohesion before mitosis. In ciliated cells, it is associated with ciliary rootlets, but in non-ciliated cells it is localized between, around and at the proximal ends of the centrioles. It dissociates from the centrioles at the onset of mitosis and re-associates with them at anaphase. Highest expression of Rootletin is found in photoreceptor cells of retina. Lower expressions have been reported in brain, trachea and kidney. In retinal photoreceptors rootlets extend from the basal bodies to the synaptic terminals and anchor endoplasmic reticulum membranes along their length. During embryonic development, it is enriched along the apical domains of neuro epithelium in brain ventricular zone, in primordia of retinal pigment epithelia, and in neural retina. Rootletin contains a globular head domain and a tail domain that consists of extended coiled-coil structures. The head domain may be required for targeting to the basal body and binding to a kinesin light chain. (Ref.: Yang, J., et al. (2002). J. Cell Biol. 159(3): 431-440).
Synonyms: Ciliary rootlet coiled-coil protein
Application: Western Blotting Analysis: A representative lot detected Rootletin in Western Blotting applications (Yang, J., et. al. (2002). J Cell Biol. 159(3):431-40).Immunohistochemistry Analysis: A representative lot detected Rootletin in Immunohistochemistry applications (Yang, J., et. al. (2002). J Cell Biol. 159(3):431-40; Sun, X., etl al. (2016). Proc Natl Acad Sci USA. 113(21):E2925-34; Yadav, S.P., et. al. (2016). Development. 143(9):1491-501; Pawlyk, B.S., et. al. (2016). Gene Ther. 23(2):196-204; Sun X., et. al. (2012). Cilia. 1(1):21; Liu, X., et. al. (2007). Proc Antl Acad Sci USA. 104(11):4413-8; Bahe, S., et. al. (2005). J Cell Biol. 171(1):27-33; Yang, J., et. al. (2005). Mol Cell Biol. 25(10):4129-37; Hong, D.H, et. al. (2003). Invest Ophthalmol Vis Sci. 44(6):2413-21).
Other Notes: Concentration: Please refer to lot specific datasheet.